IX. Mitochondrial beta-oxidation
Four enzymes and reactions
There are four individual reactions of beta-oxidation, each
catalyzed by a separate enzyme.
- Dehydrogenation between the alpha and beta carbons (C2
and C3) in a FAD-linked reaction.
- Hydration of the double bond by enoyl CoA hydratase.
- A second dehydrogenation in a NAD-linked reaction.
- Thiolytic cleavage of the thioester by beta-ketoacyl CoA
This sequence of four steps is repeated until the fatty acyl chain is completely degraded to acetyl CoA.